rnascope target probes to the dapb bacterial gene  (Advanced Cell Diagnostics Inc)

Journal: Acta Histochemica et Cytochemica

Article Title: Optimized Mouse-on-mouse Immunohistochemical Detection of Mouse ESR2 Proteins with PPZ0506 Monoclonal Antibody

doi: 10.1267/ahc.22-00043

Figure Lengend Snippet: Distribution of mouse Esr2 transcripts in ovary. The distribution of mouse Esr2 transcripts in ovary was analyzed using RNAScope in situ hybridization assays. Paraffin-embedded ovary sections were reacted with RNAScope probes against mouse Esr2 transcripts. Non-specific signals were evaluated using RNAScope Negative Control Probes (−). #, Oocyte cells; Gr, granulosa cells; T, theca cells. Bars = 200 μm in left panels and 100 μm in right panels. Similar staining patterns were obtained in three separate experiments (n = 3).

Article Snippet: Absence of non-specific signals was confirmed using a RNAScope Negative Control Probe [DapB (bacterial gene); Cat. No. 310043; Advanced Cell Diagnostics].

Techniques: RNAscope, In Situ Hybridization, Negative Control, Staining

Journal: Acta Histochemica et Cytochemica

Article Title: Optimized Mouse-on-mouse Immunohistochemical Detection of Mouse ESR2 Proteins with PPZ0506 Monoclonal Antibody

doi: 10.1267/ahc.22-00043

Figure Lengend Snippet: Distribution of mouse ESR2 proteins and Esr2 transcripts in brain subregions. Distributions of mouse ESR2 proteins and Esr2 transcripts in brain subregions were analyzed using optimized immunohistochemical staining ( A ) and RNAScope in situ hybridization ( B ) assays, respectively. ( A ) Frozen brain sections including the amygdala, BNST, and PVN were immunostained with PPZ0506. (−), Omission of primary antibody reaction. ( B ) Frozen brain sections including the amygdala, BNST, and PVN were reacted with RNAScope probes against mouse Esr2 transcripts. Non-specific signals were evaluated using RNAScope Negative Control Probes-DapB (−). 3V, third ventricle; AC, anterior commissure; BNST, bed nucleus of the stria terminalis; f, fornix; LV, lateral ventricle; opt, optic tract; PVN, paraventricular hypothalamic nucleus. Bars = 200 μm in left panels and 50 μm in middle and right panels. Similar staining patterns were obtained in three separate experiments (n = 3).

Article Snippet: Absence of non-specific signals was confirmed using a RNAScope Negative Control Probe [DapB (bacterial gene); Cat. No. 310043; Advanced Cell Diagnostics].

Techniques: Immunohistochemical staining, Staining, RNAscope, In Situ Hybridization, Negative Control

Journal: Nature Communications

Article Title: Motor neurons control blood vessel patterning in the developing spinal cord

doi: 10.1038/ncomms14583

Figure Lengend Snippet: ( a , b , e , f , i , j ) Representative images of immunostaining for Flt1 (mFlt1+sFlt1) combined with blood vessel staining (IB4 + ) at E10.5 ( a , b ), E11.5 ( e , f ) and E12.5 ( i , j ). ( c , d , g , h , k , l ). Representative images of immunostaining for Flt1 (mFlt1+sFlt1) and for Isl1/2 + (post-mitotic MNs) at E10.5 ( c , d ), E11.5 ( g , h ) and E12.5 ( k , l ). Note Flt1 can be detected in blood vessels (white arrowheads), radial glia (blue arrowheads, identified by its typical morphology), as well as in MN columns (yellow dotted outlines). ( m – p ) Representative images of RNAscope Multiplex Fluorescent Assay using specific probes to detect mFlt1 and sFlt1 combined with staining for nuclei (DAPI + ) and either staining for blood vessels (IB4 + ) ( m , o ) or immunostaining for MNs (Isl1/2 + ) ( n , p ) at E11.5 ( m , n ) and E12.5 ( o , p ). Scale bars 100 μm. Right panels: higher magnifications of insets. Scale bars of insets 10 μm.

Article Snippet: As negative control a RNAscope probe detecting dapb (bacterial gene) was used, and as positive control we used the 3-Plex-positive control RNAscope probe, targeting Polr2a , Ppib and Ubc , both provided by the manufacturer (Advanced Cell Diagnostics).

Techniques: Immunostaining, Staining, RNAscope, Multiplex Assay, Fluorescence